What are the steps for plant tissue culture?


Basic steps involved in plant tissue culture:

Preparation of suitable nutrient medium:
MS medium, White’s medium, B5 medium are few culture media. Auxins, cytokines and Gibberellins are the most commonly used growth hormones in culture medium prepared for tissue culture.

Agar: It solidifies the medium and acts as a substratum. It doesn’t have any nutritive importance. It is obtained from sea weeds and is used to provide solid surface for growth.
All these important nutrients are incorporated in the culture medium and transferred into suitable containers and then sterilized.

Selection of explant:
Explants are any excised part of the plant to be used in tissue culture like axillary buds, leaf and stem segments, root tip, shoot tip, anther, ovary and endosperm.
Always young and healthy parts of plants are selected as explants.

Sterilization of the explant:
Explants are sterilized by using different types of disinfectants like sodium hypochlorite (2%), silver nitrate (1%) calcium hypochlorite (9-10%), bromine water (1-2%), mercuric chloride (0.1-1%), hydrogen peroxide etc. and different types of antibiotics.

Inoculation of explant:
The sterile explant is inoculated on the surface of the solidified nutrient medium under aseptic (free from contamination) condition.

Laminar air flow can be used for this inoculation process.

Incubation:
Incubation is done at 25+/- 2 0C, with a relative humidity of 50-60% with 16 hours of photoperiod.
After defined period of incubation, an unorganized and undifferentiated (no root and shoot) mass of cells called callus is obtained from each explant. In some cases, root and shoot directly develop from the explant after incubation.

Transfer of callus (subculture) and regeneration:
The callus obtained from incubation of explant is transferred into another medium and reincubated.
Plantlet regenerates from the callus with the induction of roots and shoot directly from the callus.

Hardening or acclimatization:
Hardening is the gradual exposure of the plantlets for acclimatization or adjustment to the environmental conditions.

Transfer of plantlets to the field:
After the hardening process, plantlets are transferred to either the green house or the field conditions outside.

These plantlets will grow into independent and healthy plants.



https://larixconferences.com/biotechnology/

Comments

  1. MaddoxApril 28, 2025 at 11:04 PM

    Our clinic integrated PK Medi’s nurse call system last year, and it’s been a reliable communication backbone ever since. Patients feel more empowered and attended to, while our staff can manage calls more efficiently. The system logs every interaction, which helps in performance reviews and quality control.

    For More Details: https://pkmec.com/

    ReplyDelete
  2. MaddoxApril 29, 2025 at 11:07 PM

    Al-Razi Consultants connected me with a law firm that needed a nurse consultant. The job is a perfect fit and I couldn't be happier.

    For More Details: https://alraziconsultants.com/

    ReplyDelete

Post a Comment

Popular posts from this blog

What is a good starter plant and advice for a newbie who wants a little more flora in their life?

Image
Crassula ovata. The jade plant. It is a fun little plant that does best with a lot of sunlight and the occasional watering. It will even put out flowers under the right conditions. I have a few jade plants that have been with me 15ish years. Additionally, they are extremely easy to propagate. Just pull off a leaf or cut a stem and put it on dry soil. In a few weeks you might see the start of a new plant. A few pointers: 1. Don’t water too often, but water thoroughly. I usually water mine about once a week because they are i n shallow pots. If they were in a normal flower pot I might cut this down to once every two or three weeks. 2. Don't be afraid to prune. They will actually grow much faster with occasional pruning and weak fertilizer. 3. Pay attention to it. This sounds simple, but if it isn't doing well, catch it early. Try moving it to a spot with different lighting or away from a vent. While there are easier plants you could get, I still have fun with mine af...
Read more

How does the corona-virus replicate?

Image
Coronaviruses are very unusual, large, enveloped RNA viruses of both medical and veterinary importance. They have a genome of over 30,000 nucleotides (30 K bases) so can be called gigantic in virology sense . They are also slightly unusual in how they replicate themselves at the molecular level. They employ some unusual strategies to accomplish a complex program of gene expression. Coronavirus replication entails ribosome frameshifting during genome translation , the synthesis of both genomic and multiple sub genomic RNA species, and the assembly of progeny virions by a pathway that is unique among enveloped RNA viruses Coronaviruses have a two-step replication mechanism. (Many RNA virus genomes contain a single, large gene that is translated by the cellular machinery of the host to produce all viral proteins). Coronaviruses may contain up to 10 separate genes. Most ribosomes translate the biggest one of these genes, called replicase, which by itself is twice th...
Read more